Photoaffinity labeling of nucleotide binding sites with 8-azidopurine analogs: techniques and applications.

Author: Potter RL, Haley BE.

Source: Methods Enzymol.

Year: 1983

Comment:

Abstract / Excerpt:

“The thrust of this chapter is to cover the more practical aspects of labeling with the 8-azidopurine analogs as well as to emphasize the many types of biological questions that can best be answered utilizing these probes. In general they can be used to determine the cellular or subcellular distribution of purine nucleotide binding proteins. They may be used to follow changes in these proteins during cellular developmental or transformational processes, and are useful in identifying the catalytic or regulatory subunits of specific protein complexes. Structural information about the purine binding sites of proteins can also be obtained via sequencing of photolabeled binding site peptides. All of these will be discussed more fully in the sections dealing with uses of the individual analogs.”

Citation:

Potter RL, Haley BE. Photoaffinity labeling of nucleotide binding sites with 8-azidopurine analogs: techniques and applications. Methods Enzymol. 1983; 91:613-33.