Oxidation by thimerosal increases calcium levelsin renal tubular cells.

Author: Jan CR, Jiann BP, Lu YC, Chang HT, Yu CC, Chen WC, Huang JK.

Source: Pharmacol Toxicol.

Year: 2003

Comment:

Abstract / Excerpt:

“The effect of thimerosal, a reactive oxidant, on cytoplasmic free Ca2+ concentrations ([Ca2+]i) in Madin Darby canine kidney (MDCK) cells was explored by using the Ca2+-sensitive dye fura-2. Thimerosal acted in a concentration-dependent manner with an EC50 of 0.5 microM. The Ca2+ signal comprised a gradual rise and a sustained elevation. Removal of extracellular Ca2+ reduced 80% of the signal. In Ca2+-free medium, the [Ca2+]i rise induced by 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor) was completely inhibited by pretreatment with 5 microM thimerosal. The thimerosal (5 microM)-induced Ca2+ release was not changed by inhibition of phospholipase C with 2 microM U73122. Collectively, this study shows that thimerosal induced [Ca2+]i rises in renal tubular cells via releasing store Ca2+ from the endoplasmic reticulum Ca2+ stores in a manner independent of phospholipase C activity.”

Citation:

Jan CR, Jiann BP, Lu YC, et al. Oxidation by thimerosal increases calcium levelsin renal tubular cells. Pharmacol Toxicol. 2003; 93(3): 123-7.