adam

About Palczewska-Komsa M, Wilk A, Stogiera A, Chlubek D, Buczkowska-Radlińska J, Wiszniewska B.

This author has not yet filled in any details.
So far Palczewska-Komsa M, Wilk A, Stogiera A, Chlubek D, Buczkowska-Radlińska J, Wiszniewska B. has created 996 blog entries.

Animals in biomonitoring studies of environmental fluoride pollution.

Industrial development, the fluoridation of drinking water, and the use of fluoridated toothpastes all increase the emission of fluorine compounds into the environment. This leads to a significant accumulation of fluoride in living organisms, especially in bones. It is thus reasonable to monitor the level of fluoride in human and animal tissues. It can have positive or negative effects on humans and animals, though it is more commonly detrimental. Animal research on the topic has been conducted for many years and is capable of suggesting good bioindicators for environmental fluoride pollution. The aim of this study was to investigate the concentration of the fluoride ion in various tissues of invertebrates and vertebrates, including fish, amphibians, reptiles, birds, small mammals, ungulates, and predators. Additionally, this research identifies some bioindicative animal species.

Fluoride release and uptake abilities of different fissure sealants.

MATERIAL AND METHODS:
Three different fissure sealants (Fuji Triage/GC, Fissurit FX/Voco and Grandio Seal/Voco) were examined. Ten discs of each material were prepared. Each disc was incubated with distilled water and then the solution analyzed for diluted for fluoride concentration, using a combination of fluoride electrode (OrionGP 1 S/N 13824, Orion Research Inc, Boston, MA, USA) connected to an expandable ion analyzer (Orion 720A, Orion Research Inc, Boston, MA, USA). Standard curves between 1 and 100 ppm F- were used to calibrate the electrode. Cumulative fluoride release was measured on days 1, 2, 3, 5, 7, 21, 35 and 49, then two different fluoride varnishes/pastes (Profluorid Varnish/Voco, MI Paste Plus/GC), were applied to the sealants tested, and fluoride release (after reuptake) was measured on days 56, 70 and 84.

RESULTS:
Kruskal Wallis test confirmed significant differences in fluoride release between Fuji Triage/GC and Fissurit FX/Voco and Grandio Seal/Voco from day 1 (P < 0.001). The application of fluoride varnish Profluorid Varnish enhanced the fluoride release for all sealants (P < 0.05). MI Paste Plus enhanced the fluoride release for all sealants except for Fuji Triage/GC (P > 0.05).

CONCLUSIONS:
The GIC-based sealant (Fuji Triage/GC) released significantly more fluoride than the resin sealants tested. The exposure to the fluoridated varnish (Profluorid Varnish) significantly recharged the sealants tested more than the CPP-ACPF toothpaste (MI Paste Plus).

The effect of pulsed electromagnetic fields on microleakage of amalgam restorations: an in vitro study.

Background: Previous studies have reported an increase in the mercury release from dental amalgam restorations, following exposure to electromagnetic fields generated by sources such as mobile phones and magnetic resonance imaging (MRI). It has also been shown that MRI increases microleakage of amalgam restorations. In this study, Helmholtz coils are used for generating pulsed electromagnetic fields (PEMF).

Objectives: The purpose of this study was to evaluate the effect of PEMFs on microleakage of amalgam restorations, using a pair of Helmholtz coils.
Patients and Methods: Standardized class V cavities were prepared on the facial surfaces of 46 non-carious extracted human premolars. Then, the samples were randomly divided into experimental and control groups, each containing 23 teeth. The experimental group was exposed to the uniform magnetic fields generated by a pair helmholtz coils. The magnetic field strength at the central point of the two coils was 0.1 mT. All specimens were placed in 2% basic fuchsin solution. Then the teeth were sectioned, examined under a stereomicroscope, and scored for microleakage according to the degree of dye penetration.

Results: There was no significant difference between the two groups regarding the microleakage score.
Conclusions: The results of the present study suggest that PEMF exposure does not have adverse effects on microleakage of amalgam restorations.

Mercury release of amalgams with various silver contents after exposure to bleaching agent.

“BACKGROUND:
Since it is possible for carbamide peroxide (CP) bleaching agent to contact old amalgam restorations, the present in vitro study evaluated the amount of dissolved mercury released from amalgam restorations with various percent-ages of silver content subsequent to the use of 15% CP.

METHODS:
Thirty ANA 2000 amalgam disks with 43.1% silver content and thirty ANA 70 amalgam disks with 69.3% silver content were prepared. In each group, 15 samples were randomly placed in glass tubes containing 15% CP (as experimental groups) and the remaining 15 samples were placed in buffered phosphate solution (as control groups) with the same 3-mL volume for 48 hours. Subsequently, the amount of mercury dissolved in each test tube was measured using Mercury Analyzing System (Cold Vapor Atomic Absorption, MASLO, Shimadzu, Japan). Data was analyzed with two-way ANOVA and a post hoc Tukey test. (α = 0.05).

RESULTS:
The amount of mercury released after exposure to CP was significantly higher than that released after exposure to buffered phosphate (P

Dose-response analysis indicating time-dependent neurotoxicity caused by organic and inorganic mercury—Implications for toxic effects in the developing brain.

A latency period preceding neurotoxicity is a common characteristic in the dose-response relationship induced by organic mercury. Latency periods have typically been observed with genotoxicants in carcinogenesis, with cancer being manifested a long time after the initiating event. These observations indicate that even a very small dose may cause extensive adverse effects later in life, so the toxicity of the genotoxic compound is dose and time-dependent. In children, methylmercury exposure during pregnancy (in utero) has been associated with delays in reaching developmental milestones (e.g., age at first walking) and decreases in intelligence, increasing in severity with increasing exposure. Ethylmercury exposure from thimerosal in some vaccines has been associated, in some studies, with autism and other neurological disorders in children. In this paper, we have examined whether dose-response data from in vitro and in vivo organic mercury toxicity studies fit the Druckrey-Küpfmüller equation c·t(n)=constant (c=exposure concentration, t=latency period), first established for genotoxic carcinogens, and whether or not irreversible effects are enhanced by time of exposure (n≥1), or else toxic effects are dose-dependent while time has only minor influence on the adverse outcome (n<1). The mode of action underlying time-dependent toxicity is irreversible binding to critical receptors causing adverse and cumulative effects. The results indicate that the Druckrey-Küpfmüller equation describes well the dose-response characteristics of organic mercury induced neurotoxic effects. This amounts to a paradigm shift in chemical risk assessment of mercurial compounds and highlights that it is vital to perform toxicity testing geared to investigate time-dependent effects.

By |2018-07-29T17:39:06+00:00January 1st, 2016|Mercury|

Assessment of oxidative stress induced by various restorative materials: an in vivo biochemical study.

Background: To determine in vivo oxidative stresses induced by dental amalgam, composite resin, and glass ionomer cement (GIC).
Materials and Methods: A total of 60 patients were selected for the study between the ages of 15 and 40 years. Informed consent and Institutional Ethical Approval was obtained. The samples were divided into three groups depending on the type of restorative material planned for them, i.e., silver amalgam, composite resin, and GIC. Saliva was collected before restoration, 24 h, 7 days, and 14 days after restoration. The salivary oxidative stress malondialdehyde (MDA) was measured and statistically analyzed using Mann–Whitney test, Kruskal–Wallis test, and Friedman’s test.

Results: The results show that the patients treated with amalgam restoration had shown significantly higher values as compared to composite and GIC at all the three-time intervals studied.

Conclusion: This study revealed that amalgam fillings were associated with the highest oxidative stress marker MDA as compared with composite and glass ionomer restoration at all the three-time intervals studied. Even after 14 days, the values were much higher indicating free ion leaching from the restoration.

Dental amalgam exposure can elevate urinary mercury concentrations in children.

“METHODS:
In this longitudinal study, 463, 367 and 348 children, 8-11 years of age, were evaluated at baseline, and at the first and second follow-up visits, respectively. The interval between each survey was 6 months. For the oral examination and urine sample, the amalgam-filled tooth surface (TS), and U-Hg and creatinine concentrations of participants were determined, and the cumulative amalgam-filled TS and cumulative creatinine-adjusted U-Hg were calculated. To assess potential covariates, socio-demographic factors, oral health behaviour and dietary factors were surveyed by questionnaire. Data were analysed by the t-test, correlation analysis and mixed-model analysis. The statistical analyses were performed using SPSS 18.0.

RESULTS:
Children with more than one amalgam-filled TS exhibited significantly higher creatinine-adjusted U-Hg concentrations than those without, in all three survey periods (P

Oral lichenoid reactions, patch tests, and mercury dental amalgam.

The findings of Suter and Warnakulasuriya (2015) are very promising for those interested in the underlying pathogenesis of amalgam-related oral lichenoid reactions (OLRs) (1). However, we would like to underscore the importance of clinical relevance of patch test reactions in support of the causal relation between oral lichenoid reactions and dental metal allergy (2). To achieve this goal, in our view, it is necessary to extend the ‘mercury-containing dental amalgam series of patch tests’.

By |2018-07-29T17:31:56+00:00January 1st, 2016|Mercury|

Fluoride content in alcoholic drinks.

The aim of the study was to determine the role of alcoholic drinks as a potential source of dietary fluoride by means of measuring fluoride levels in selected alcoholic drinks available on the Polish market that are also diverse in terms of the percentage content of ethanol. The study was conducted on 48 types of drinks with low, medium, and high alcohol content available on the Polish market and offered by various manufacturers, both Polish and foreign. Fluoride concentrations in individual samples were measured by potentiometric method with a fluoride ion-selective electrode. The highest fluoride levels were determined in the lowest percentage drinks (less than 10 % v/v ethanol), with the lowest fluoride levels observed in the highest percentage drinks (above 40 % v/v ethanol). In terms of types of alcoholic drinks, the highest fluoride levels were determined in beers and wines, while the lowest levels were observed in vodkas. These data confirm the fact that alcoholic beverages need to be considered as a significant source of fluoride delivered into the body.

Increased mercury release due to exposure to electromagnetic radiation as a limiting factor for using dental amalgam.

Background: Although it is one of the most toxic nonradioactive elements, mercury is widely  used in dental amalgam. Mercury is a toxic element which can damage various organs such  as central nervous system, renal, respiratory and hematologic systems. The adverse health impacts associated to exposure to some common sources of electromagnetic fields including  laptop computers, mobile phones, MRI and mobile phone jammers have been evaluated by our laboratory in our previous investigations. In this study, we aimed to evaluate the effect of of X ray exposure on microleakage of amalgam restoration. Materials and Methods: Standardized class V cavities were prepared on the buccal surfaces of 46 non-carious freshly extracted human premolars. The teeth were randomly divided into experimental and control groups. Experimental group were exposed to X-ray using an intraoral radiography machine at 60 kVp, 0.1 s, 7 mA with 2.5 mm Al total filtration. The absorbed dose was 245.0 ± 0.5 µGy. All specimens were placed in 2 % basic fuchsin solution for 24 hours. Then the specimens were sectioned and microleakage was assessed according to dye penetration using a stereomicroscope. Statistical analysis was performed with the Mann-Whitney U-test. Results: Microleakage was significantly higher in the X-ray exposed teeth compared to those of the non-irradiated samples. Conclusion: The results of the present study suggest that X-ray exposure increased microleakage of amalgam restorations.

Go to Top