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Chronic bacterial and viral infections in neurodegenerative and neurobehavioral diseases.

Often, patients with neurodegenerative or neurobehavioral diseases have chronic, neuropathic infections that could be important in disease inception, disease progression, or increasing the types or severities of signs and symptoms. Although controversial, the majority of patients with various neurodegenerative or neurobehavioral conditions, such as amyotrophic lateral sclerosis, multiple sclerosis, Alzheimer’s disease, Parkinson’s disease, and autistic spectrum disorders, show evidence of central nervous system or systemic  bacterial and viral infections. For example, using serology or polymerase chain reaction evidence of Chlamydia pneumoniae, Borrelia burgdorferi, Mycoplasma species, human herpesvirus-1 and -6, and other bacterial and viral infections revealed high infection rates that were not found in control subjects.

By |2018-07-27T00:10:00+00:00January 1st, 2008|Other|

The biocompatibility of resin-modified glass-ionomer cements for dentistry.

OBJECTIVES:
The biological effects of resin-modified glass-ionomer cements as used in clinical dentistry are described, and the literature reviewed on this topic.

METHODS:
Information on resin-modified glass-ionomers and on 2-hydroxyethyl methacrylate (HEMA), the most damaging substance released by these materials, has been collected from over 50 published papers. These were mainly identified through Scopus.

RESULTS:
HEMA is known to be released from these materials and has a variety of damaging biological properties, ranging from pulpal inflammation to allergic contact dermatitis. These are therefore potential hazards from resin-modified glass-ionomers. However, clinical results with these materials that have been reported to date are generally positive.

CONCLUSIONS/SIGNIFICANCE:
Resin-modified glass-ionomers cannot be considered biocompatible to nearly the same extent as conventional glass-ionomers. Care needs to be taken with regard to their use in dentistry and, in particular, dental personnel may be at risk from adverse effects such as contact dermatitis and other immunological responses.

By |2018-07-26T23:43:49+00:00January 1st, 2008|Fluoride|

Lesions of endodontic origin and risk of coronary heart disease

A paucity of epidemiologic research exists regarding systemic health consequences of endodontic disease. This study evaluated whether incident radiographically evident lesions of endodontic origin were related to development of coronary heart disease (CHD) among 708 male participants in the VA Dental Longitudinal Study. At baseline and every three years for up to 32 years, participants (who were not VA patients) received complete medical and dental examinations, including full-mouth radiographs. Cox regression models estimated the relationship between incident lesions of endodontic origin and time to CHD diagnosis. Among those < or = 40 years old, incident lesions of endodontic origin were significantly associated with time to CHD diagnosis (p < 0.05), after adjustment for covariates of interest, with hazard ratios decreasing as age increased. Among those > 40 years old, no statistically significant association was observed. These findings are consistent with research that suggests relationships between chronic periodontal inflammation and the development of CHD, especially among younger men.

Comparison of nanoparticle filtration performance of NIOSH-approved and CE-marked particulate filtering facepiece respirators

The National Institute for Occupational Safety and Health (NIOSH) and European Norms (ENs) employ different test protocols for evaluation of air-purifying particulate respirators commonly referred to as filtering facepiece respirators (FFR). The relative performance of the NIOSH-approved and EN-certified ‘Conformité Européen’ (CE)-marked FFR is not well studied. NIOSH requires a minimum of 95 and 99.97% efficiencies for N95 and P100 FFR, respectively; meanwhile, the EN requires 94 and 99% efficiencies for FFRs, class P2 (FFP2) and class P3 (FFP3), respectively. To better understand the filtration performance of NIOSH- and CE-marked FFRs, initial penetration levels of N95, P100, FFP2 and FFP3 respirators were measured using a series of polydisperse and monodisperse aerosol test methods and compared. Initial penetration levels of polydisperse NaCl aerosols [mass median diameter (MMD) of 238 nm] were measured using a method similar to the NIOSH respirator certification test method. Monodisperse aerosol penetrations were measured using silver particles for 4-30 nm and NaCl particles for 20-400 nm ranges. Two models for each FFR type were selected and five samples from each model were tested against charge neutralized aerosol particles at 85 l min(-1) flow rate. Penetrations from the 238 nm MMD polydisperse aerosol test were <1% for N95 and FFP2 models and <0.03% for P100 and FFP3 models. Monodisperse aerosol penetration levels showed that the most penetrating particle size (MPPS) was in the 30-60 nm range for all models of FFRs tested in the study. Percentage penetrations at the MPPS were <4.28, <2.22, <0.009 and <0.164 for the N95, FFP2, P100 and FFP3 respirator models, respectively. The MPPS obtained for all four FFR types suggested particle capturing by electrostatic mechanism. Liquid isopropanol treatment of FFRs shifted the MPPS to 200-300 nm and dramatically increased polydisperse as well as monodisperse aerosol penetrations of all four FFR types indicating that all the four FFR types share filtration characteristics of electret filters. Electrostatic charge removal from all four FFR types also increased penetration levels of 400-1000 nm range particles. Particle penetration data obtained in this study showed that the eight models of NIOSH-approved N95 and P100 and CE-marked FFP2 and FFP3 respirators used in this study provided expected levels of laboratory filtration performance against nanoparticles.

By |2021-01-20T00:05:58+00:00January 1st, 2008|Other|

Interactions of human commensal bacteria with amalgam derived mercury: the science and its implications for infectious disease and neurotoxicology.

In summary, there is currently a renaissance of interest in the contributions of the commensal microbiota to many aspects of health and disease from digestion to the development of the immune system and even to effects on the central nervous system (Yan and Polk, 2004; Kanauchi et al., 2005; Macdonald and Monteleone, 2005). One traditional area upon which this new perspective has yet to impinge is toxicology. The vigorous metabolism of all forms of Hg by bacterial members of the commensal microbiota is a rich example of what our small fellow travelers can marshal for their defense against the toxic agents to which we expose ourselves. Hopefully, toxicologists , especially those concerned with the widespread exposure to Hg from amalgam restorations, will begin to collaborate with microbiologists to take into account these bacterial processes (Fig. 1) in understanding the risks of amalgams and devising means to protect those with them and those having them removed.

By |2018-07-31T22:19:02+00:00January 1st, 2008|Mercury|

Effects of a stannous fluoride-impregnated dental floss on in vivo salivary fluoride levels.

PURPOSE:
The aim of this in vivo pilot study was to determine the concentration of fluoride retained intra-orally in saliva after flossing with dental floss impregnated with stannous fluoride (SnF(2)).

METHODS:
Participants flossed their teeth ad libitum with 2 premeasured lengths of fluoridated dental floss. Expectorated saliva samples were collected in vials before flossing (PF), immediately postflossing(IPF), at 30 minutes (30), and 1 hour (60) after flossing for analysis with a fluoride-specific electrode and an Orion millivoltmeter. Postflossing samples were compared to the preflossing samples using ANOVA and Tukey’s HSD.

RESULTS:
Differences between the PF and IPF group means were found to be statistically significant at p<0.01. No other significant differences were found between or among any of the groups. Salivary fluoride levels at 60 minutes (60) were similar to those prior to flossing (PF).

CONCLUSIONS:
It can be concluded that fluoride can be released from flossing with the tested SnF(2)-impregnated dental floss elevating salivary fluoride levels for at least 30 minutes. Use of this fluoride-containing dental floss offers an option for delivery of fluoride to individuals at risk for dental caries.

By |2018-07-20T21:13:59+00:00January 1st, 2008|Fluoride|

Populations receiving optimally fluoridated public drinking water–United States, 1992-2006.

Water fluoridation has been identified by CDC as one of 10 great public health achievements of the 20th century. The decline in the prevalence and severity of dental caries (tooth decay) in the United States during the past 60 years has been attributed largely to the increased use of fluoride. Community water fluoridation is an equitable and cost-effective method for delivering fluoride to the community. A Healthy People 2010 objective is to increase to 75% the proportion of the U.S. population served by community water systems who receive optimally fluoridated water. To update and revise previous reports on fluoridation in the United States and describe progress toward the Healthy People 2010 objective, CDC analyzed fluoridation data for the period 1992-2006 from the 50 states and District of Columbia (DC). The results indicated that the percentage of the U.S. population served by community water systems who received optimally fluoridated water increased from 62.1% in 1992, to 65.0% in 2000, and 69.2% in 2006, and those percentages varied substantially by state. Public health officials and policymakers in states with lower percentages of residents receiving optimal water fluoridation should consider increasing their efforts to promote fluoridation of community water systems to prevent dental caries.

By |2018-07-19T20:09:47+00:00January 1st, 2008|Fluoride|

On approximal caries prevention using fluoridated toothpicks, dental floss and interdental brushes.

Material and methods and Results:

The F release of 26 brands of toothpicks and floss was followed for 24 hrs in vitro. A large variation in the release between these products was found; in general, toothpicks resulted in larger amounts of F compared with floss. The release in vivo was studied using single and multiple fluoridated toothpicks and dental floss, as well as in combination with toothbrushing or a mouthrinse with 0.2% NaF. Moreover, the administration of F by an interdental brush dipped in 0.2% NaF gel (here called the “Inter Dental Brush Gel Method”) was evaluated. Approximal saliva was collected, using paper points, before and up to 60 min after treatment. Both toothpicks and floss resulted in enhanced F concentrations in vivo. An interdental brush dipped in 0.2% NaF gel and a mouthrinse with 0.2% NaF resulted in the same F concentration as after using multiple toothpicks. All combinations of toothpicks and dental floss with F rinsing resulted in higher concentrations than after only toothbrushing or in combination with brushing. The most optimal order was to use toothpicks and dental floss after toothbrushing and before rinsing. Fifteen adults with full dentures, in which demineralised enamel and dentine specimens had been mounted, were included in an in situ experimental caries model. Toothpicks or floss, impregnated with NaF and amine fluoride (AmF), were used regularly for four weeks. All the products inhibited continuous demineralisation – dental floss somewhat more than toothpicks. A reduction in plaque micro-organisms was also found. Recommendations made by dental staff in relation to approximal cleaning aids were evaluated by a questionnaire sent to 500 dentists, 500 dental hygienists and 1000 patients in Sweden. The ability to remove approximal plaque was also evaluated in 60 regular users of approximal aids. Recommendations by dental staff are mostly given to children and adolescents in order to prevent dental caries and to older individuals to prevent gingivitis and periodontal disease. Approximal plaque appears to be more easily removed by regular users of interdental brushes compared with the use of toothpicks and dental floss.

By |2018-07-30T20:04:45+00:00January 1st, 2008|Fluoride|

Fluorosis in horses drinking artificially fluoridated water.

Quarter Horses drinking water artificially fluoridated at 0.9 to 1.1 ppm over long periods of time developed dental fluorosis. Even when the horses had not been exposed to artificially fluoridated water (AFW) during formation of enamel, brown discoloration occurred and progressed. Pronounced loss of tooth-supporting alveolar bone with recession of bone and gingiva was also present as more severe signs of chronic fluorosis than enamel changes alone. The scene of these events was a lowland part of Texas just above sea level. AFW at these low concentrations can obviously induce chronic fluorosis in its own right without the support of high altitude. With a change to low fluoride (0.1 ppm) well water, a remarkable improvement was observed in the general health of the horses in a very short time. Most notably, bothersome episodes of colic promptly ceased.

By |2018-07-25T19:50:07+00:00January 1st, 2008|Fluoride|

Reductive defluorination of perfluorooctane sulfonate.

Perfluorooctane sulfonate (PFOS) is under increased scrutiny as an environmental pollutant due to recent reports of its worldwide distribution, environmental persistence, and bioaccumulation potential. The susceptibility of technical PFOS and PFOS branched isomers to chemical reductive dehalogenation with vitamin B12 (260 microM) as catalyst and Ti(III)-citrate (36 mM) as bulk reductant in anoxic aqueous solution at 70 degrees C and pH 9 was evaluated in this study. Defluorination was confirmed by fluoride release measurements of 18% in technical PFOS, equivalent to the removal 3 mol F-/mol PFOS, and 71% in PFOS branched isomers equivalent to the removal of 12 mol F-/mol PFOS. Degradation of PFOS was further confirmed by monitoring the disappearance of PFOS compounds with reaction time by suppressed conductivity ion chromatography, LC-MS/MS, and 19F NMR studies. The PFOS compounds differed in their susceptibility to reductive degradation by vitamin B12Ti(III) citrate. Chromatographic peaks corresponding to branched PFOS isomers disappeared whereas the peak corresponding to linear PFOS was stable. To our knowledge this is the first report of reductive dehalogenation of PFOS catalyzed by a biomolecule.

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