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Effects of a stannous fluoride-impregnated dental floss on in vivo salivary fluoride levels.

PURPOSE:
The aim of this in vivo pilot study was to determine the concentration of fluoride retained intra-orally in saliva after flossing with dental floss impregnated with stannous fluoride (SnF(2)).

METHODS:
Participants flossed their teeth ad libitum with 2 premeasured lengths of fluoridated dental floss. Expectorated saliva samples were collected in vials before flossing (PF), immediately postflossing(IPF), at 30 minutes (30), and 1 hour (60) after flossing for analysis with a fluoride-specific electrode and an Orion millivoltmeter. Postflossing samples were compared to the preflossing samples using ANOVA and Tukey’s HSD.

RESULTS:
Differences between the PF and IPF group means were found to be statistically significant at p<0.01. No other significant differences were found between or among any of the groups. Salivary fluoride levels at 60 minutes (60) were similar to those prior to flossing (PF).

CONCLUSIONS:
It can be concluded that fluoride can be released from flossing with the tested SnF(2)-impregnated dental floss elevating salivary fluoride levels for at least 30 minutes. Use of this fluoride-containing dental floss offers an option for delivery of fluoride to individuals at risk for dental caries.

By |2018-07-20T21:13:59+00:00January 1st, 2008|Fluoride|

Populations receiving optimally fluoridated public drinking water–United States, 1992-2006.

Water fluoridation has been identified by CDC as one of 10 great public health achievements of the 20th century. The decline in the prevalence and severity of dental caries (tooth decay) in the United States during the past 60 years has been attributed largely to the increased use of fluoride. Community water fluoridation is an equitable and cost-effective method for delivering fluoride to the community. A Healthy People 2010 objective is to increase to 75% the proportion of the U.S. population served by community water systems who receive optimally fluoridated water. To update and revise previous reports on fluoridation in the United States and describe progress toward the Healthy People 2010 objective, CDC analyzed fluoridation data for the period 1992-2006 from the 50 states and District of Columbia (DC). The results indicated that the percentage of the U.S. population served by community water systems who received optimally fluoridated water increased from 62.1% in 1992, to 65.0% in 2000, and 69.2% in 2006, and those percentages varied substantially by state. Public health officials and policymakers in states with lower percentages of residents receiving optimal water fluoridation should consider increasing their efforts to promote fluoridation of community water systems to prevent dental caries.

By |2018-07-19T20:09:47+00:00January 1st, 2008|Fluoride|

On approximal caries prevention using fluoridated toothpicks, dental floss and interdental brushes.

Material and methods and Results:

The F release of 26 brands of toothpicks and floss was followed for 24 hrs in vitro. A large variation in the release between these products was found; in general, toothpicks resulted in larger amounts of F compared with floss. The release in vivo was studied using single and multiple fluoridated toothpicks and dental floss, as well as in combination with toothbrushing or a mouthrinse with 0.2% NaF. Moreover, the administration of F by an interdental brush dipped in 0.2% NaF gel (here called the “Inter Dental Brush Gel Method”) was evaluated. Approximal saliva was collected, using paper points, before and up to 60 min after treatment. Both toothpicks and floss resulted in enhanced F concentrations in vivo. An interdental brush dipped in 0.2% NaF gel and a mouthrinse with 0.2% NaF resulted in the same F concentration as after using multiple toothpicks. All combinations of toothpicks and dental floss with F rinsing resulted in higher concentrations than after only toothbrushing or in combination with brushing. The most optimal order was to use toothpicks and dental floss after toothbrushing and before rinsing. Fifteen adults with full dentures, in which demineralised enamel and dentine specimens had been mounted, were included in an in situ experimental caries model. Toothpicks or floss, impregnated with NaF and amine fluoride (AmF), were used regularly for four weeks. All the products inhibited continuous demineralisation – dental floss somewhat more than toothpicks. A reduction in plaque micro-organisms was also found. Recommendations made by dental staff in relation to approximal cleaning aids were evaluated by a questionnaire sent to 500 dentists, 500 dental hygienists and 1000 patients in Sweden. The ability to remove approximal plaque was also evaluated in 60 regular users of approximal aids. Recommendations by dental staff are mostly given to children and adolescents in order to prevent dental caries and to older individuals to prevent gingivitis and periodontal disease. Approximal plaque appears to be more easily removed by regular users of interdental brushes compared with the use of toothpicks and dental floss.

By |2018-07-30T20:04:45+00:00January 1st, 2008|Fluoride|

Fluorosis in horses drinking artificially fluoridated water.

Quarter Horses drinking water artificially fluoridated at 0.9 to 1.1 ppm over long periods of time developed dental fluorosis. Even when the horses had not been exposed to artificially fluoridated water (AFW) during formation of enamel, brown discoloration occurred and progressed. Pronounced loss of tooth-supporting alveolar bone with recession of bone and gingiva was also present as more severe signs of chronic fluorosis than enamel changes alone. The scene of these events was a lowland part of Texas just above sea level. AFW at these low concentrations can obviously induce chronic fluorosis in its own right without the support of high altitude. With a change to low fluoride (0.1 ppm) well water, a remarkable improvement was observed in the general health of the horses in a very short time. Most notably, bothersome episodes of colic promptly ceased.

By |2018-07-25T19:50:07+00:00January 1st, 2008|Fluoride|

Reductive defluorination of perfluorooctane sulfonate.

Perfluorooctane sulfonate (PFOS) is under increased scrutiny as an environmental pollutant due to recent reports of its worldwide distribution, environmental persistence, and bioaccumulation potential. The susceptibility of technical PFOS and PFOS branched isomers to chemical reductive dehalogenation with vitamin B12 (260 microM) as catalyst and Ti(III)-citrate (36 mM) as bulk reductant in anoxic aqueous solution at 70 degrees C and pH 9 was evaluated in this study. Defluorination was confirmed by fluoride release measurements of 18% in technical PFOS, equivalent to the removal 3 mol F-/mol PFOS, and 71% in PFOS branched isomers equivalent to the removal of 12 mol F-/mol PFOS. Degradation of PFOS was further confirmed by monitoring the disappearance of PFOS compounds with reaction time by suppressed conductivity ion chromatography, LC-MS/MS, and 19F NMR studies. The PFOS compounds differed in their susceptibility to reductive degradation by vitamin B12Ti(III) citrate. Chromatographic peaks corresponding to branched PFOS isomers disappeared whereas the peak corresponding to linear PFOS was stable. To our knowledge this is the first report of reductive dehalogenation of PFOS catalyzed by a biomolecule.

Legislation and informed consent brochures for dental patients receiving amalgam restorations.

In 2008, Norway banned the use of mercury for amalgam restorations. Four states in the United States have developed In-formed Consent Brochures for amalgam restorations that must be given to their dental patients. The authors describe a patient who had a large cavity in his left lower molar tooth no.18 that had to be removed by an oral surgeon. When the patient went to the oral surgeon, the surgeon told the patient that he would replace the carious tooth with a gold implant. He was not given an Informed Consent Brochure regarding dental restorative materials. The oral surgeon extracted the carious tooth, replacing the tooth with a sup-posed gold crown implant. On his yearly dental examination, his dentist took an x-ray of his dental implant and explained that the x-ray could not distinguish whether the implant contained either gold or mercury. Consequently, the dentist referred him to a dental clinic in which the dental implant could be removed without mer-cury contamination of the patient’s neurologic system during the extraction of the implant from the root canal. During the removal of the dental restoration, the dentist found build up expanding into the root canal that had a black color. The crown and underlying tooth were sent to ALT BioScience for analysis. Elemental analy-sis of the crown and underlying tooth confirmed the presence of mercury in the restoration. The patient should have been given an Informed Consent Brochure by the dentist that described the dental restoration that was used in the dental implant.

Effect of mercury (II) on Nrf2, thioredoxin reductase-1 and thioredoxin-1 in human monocytes.

OBJECTIVES:
Human blood levels of mercury are commonly 10nM, but may transiently reach 50-75nM after dental amalgam placement or removal. Controversy persists about the use of mercury because the effects of these ‘trace’ levels of mercury are not clear. Concentrations of mercury > or =5000nM unequivocally alter redox balance in blood cells including monocytes. In the current study, we tested a hypothesis that concentrations of mercury <100nM altered levels and activities of key proteins that maintain monocytic redox balance.

METHODS:
Human THP1 monocytes were exposed to 10-75nM of Hg(II) for 6-72h, with or without activation by lipopolysaccharide (LPS). The redox management proteins Nrf2 and thioredoxin-1 (Trx1) were separated by electrophoresis, then quantified by immunoblotting. The activity of the seleno-enzyme thioredoxin reductase (TrxR1), important in maintaining Trx1 redox balance, was measured by cell-free and cell-dependent assays.

RESULTS:
Concentrations of Hg(II) between 10-75nM increased Nrf2 levels (3.5-4.5 fold) and decreased Trx1 levels (2-3 fold), but these changes persisted <24h. Hg(II) potently inhibited (at concentrations of 5-50nM) TrxR1 activity in both cell-free and intracellular assays. Furthermore, Hg(II) transiently amplified LPS-induced Nrf2 levels by 2-3 fold and limited LPS-induced decreases in Trx1. All effects of Hg(II) were mitigated by pre-adding N-acetyl-cysteine (NAC) or sodium selenide (Na2SeO3), supplements of cellular thiols and selenols, respectively.

SIGNIFICANCE:
Our results suggest that nanomolar concentrations of Hg(II) transiently alter cellular redox balance in monocytes that trigger changes in Nrf2 and Trx1 levels. These changes indicate that monocytes have a capacity to adapt to trace concentrations of Hg(II) that are introduced into the bloodstream after dental amalgam procedures or fish consumption. The ability of monocytes to adapt suggests that low levels of mercury exposure from dental amalgam may not overtly compromise monocyte function.

Estimating consumer exposure to PFOS and PFOA.

Perfluorinated compounds have been used for more than 50 years as process aids, surfactants, and for surface protection. This study is a comprehensive assessment of consumer exposure to perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) from a variety of environmental and product-related sources. To identify relevant pathways leading to consumer exposure to PFOS and PFOA a scenario-based approach has been applied. Scenarios represent realistic situations where age- and gender-specific exposure occurs in the everyday life of consumers. We find that North American and European consumers are likely to experience ubiquitous and long-term uptake doses of PFOS and PFOA in the range of 3 to 220 ng per kg body weight per day (ng/kg(bw)/day) and 1 to 130 ng/kg(bw)/day, respectively. The greatest portion of the chronic exposure to PFOS and PFOA is likely to result from the intake of contaminated foods, including drinking water. Consumer products cause a minor portion of the consumer exposure to PFOS and PFOA. Of these, it is mainly impregnation sprays, treated carpets in homes, and coated food contact materials that may lead to consumer exposure to PFOS and PFOA. Children tend to experience higher total uptake doses (on a body weight basis) than teenagers and adults because of higher relative uptake via food consumption and hand-to-mouth transfer of chemical from treated carpets and ingestion of dust. The uptake estimates based on scenarios are within the range of values derived from blood serum data by applying a one-compartment pharmacokinetic model.

Implication of immune interactions in bacterial virulence: is Porphyromonas gingivalis an “Invader” or “Stealth Element” in periodontal lesions?

Porphyromonas gingivalis (Pg), a Gram-negative anaerobic black-pigmented rod bacterium, has been recognized as the most potent etiologic bacte-rium in human chronic periodontitis. It possesses a variety of putative virulence factors providing both tissue destruction and host evasion including lipopolysac-charides (LPS), fi mbriae, various proteinases, etc. These factors actively participate in periodontal tissue destruction. However, recent evidence suggests that Pg has also evolved mechanisms to inhibit or confuse host immune systems. Thus, Pg is suggested to behave not only like an “active invader”, but also like a “stealth element” in periodontal lesions. In the present study, repeated exposure of Pg components induced tolerance resulting in selective inhibition of cytokine produc-tion of both monocytes and gingival fi broblasts in a different fashion from that described for LPS in Escherichia coli. It was also revealed that Pg LPS induced a unique dendritic cell subset with a CD14+CD16+ phenotype that exhibited weak maturation. In animal studies, administration of live Pg or its LPS exerted a regula-tory effect on systemic markers such as triglycerides or adiponectin. Taken together, these fi ndings suggest that Pg may be able to adapt to the local immune defense, contributing to the connection between systemic and periodontal disease.

By |2018-07-30T23:35:22+00:00January 1st, 2007|Periodontal Disease|
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